One-Step RT-PCR Master BlueMix is a premixed lyophilized reagent for RT-PCR efficient amplification, which includes double-enclosed hot-start Super HTaq

DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl 2 ,dNTPs and bromophenol blue electrophoresis indicator. When using, it is only necessary to add RNA templates and primers (or probes) according to the required amount, and add PCR grade water until the volume of each reaction is 20 µL, and then the reverse transcription and PCR amplification reactions of RNAsamples can be started.

The 3' end of the PCR product amplified by this product has an "A" base attached, so it can be directly cloned into T-Vector; the PCR product is also suitable for sequencing identification;This product contains blue Loading Buffer dye for DNA sample electrophoresis tracing, PCR products can be directly used for agarose gel electrophoresis, easy to use.Bromophenol blue dye is a standard electrophoretic tracer dye with a band size of about 300 bp in 1% agarose gel

Simple

• Easy operation, suitable for RNA or DNA samples;

Specificity and Sensitivity

•  Specifically double-blocked anti-taq antibody, Hot-Start Taq DNA polymerase for highly specific amplification and quantitation, tolerant to PCR inhibitors;

Convenient

• Lyophilized reagent, one tube for one reaction.