.One-Step RT-PCR Master Mix (lyophilized) is a premixed lyophilized reagent for RT-PCR efficient amplification, which includes double-enclosed hot-start Super
HTaq DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl 2 and dNTPs. When using, it is only necessary to add RNA templates and primers (or
probes) according to the required amount, and add PCR grade water until the volume of each reaction is 20 µL, and then the reverse transcription and PCR
amplification reactions of RNAsamples can be started.
The 3' end of the PCR product amplified by this product has an "A" base attached, so it can be directly cloned into T-Vector; the PCR product is also suitable for
sequencing identification.
Simple
Easy operation, suitable for RNA or DNA samples;
Specificity and Sensitivity
Specifically double-blocked anti-taq antibody,
Hot-Start Taq DNA polymerase for highly specific amplification and quantitation,
tolerant to PCR inhibitors;
Lyophilized
Products without glycerol can be used for lyophilized reagents.
Component | Specifications | Note |
4× One-step RT-qPCR Master Mix | 1mL | with glycerol |
4× One-step RT-qPCR Master Mix | 5mL | with glycerol |
