Ready-to-Use PCR Master Mix (lyophilized) is a premixed lyophilized reagent for PCR efficient amplification, which includes double-enclosed hot-start Super HTaq DNA polymerase, MgCl2 and dNTPs. When using, only add DNA templates and primers (or probes) according to the required amount, and add PCR-grade water until each reaction reaches 20µL, and then the PCR amplification reaction of the DNA sample can be started. 

The 3' end of the PCR product amplified by this product has an "A" base attached, so it can be directly cloned into T-Vector; the PCR product is also suitable for sequencing identification.

Freeze-dried and stable

•  freeze-dried reagents can be transported at room temperature and stored at room temperature, with a validity period of 2 years.

Convenient operation

•  reduce the PCR system configuration process and complete the experiment quickly.

Cost-saving

•  freeze-dried pellets are pre-packed in eight-link PCR tubes and can be used directly. If the single use amount is less than 8 tests, the PCR tube can be cut to recycle the unused part to save reagents.

Efficient and accurate

•  using double-blocked hot start Taq DNA polymerase, its polymerase activity is inhibited before heating to high temperature, so it can inhibit non-specific amplification caused by primer dimers under normal temperature conditions, ensuring efficient and accurate PCR reactions.