Intended Use
Product features
Basic parameters
Intended Use
This product is obtained by isolating the reverse transcriptase gene from Moloney Murine Leukaemia Virus (MMLV) in Escherichia coli after expression and purification for several times. It has the same function as natural MMLV, and it is a DNA polymerase with RNA-dependent enzyme, which is widely used in the synthesis of 1st-Strand cDNA, and it lacks the activity of RNase H, so it can be used for the synthesis of longer cDNAs.
Product features
Convenience:
Compatible with one- and two-step RT-PCR reactions.
RNaseH-:
RNA template suitable for complex secondary structures and has higher thermal stability.
Strong synthesis ability:
synthetic fragment ≤8kb.
Basic parameters
| Component | Specifications | |
| M-MLV Reverse transcriptase（RNase H-） | 50uL | 5 x 50uL |
| 5 x RT buffer | 2 x 1mL | 2 x 5mL |
