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HP Taq DNA Polymerase
Product Introduction
This product is a hot-start enzyme that is genetically modified based on ordinary polymerase, and has added protective agents, making it highly tolerant to PCR inhibitors such as EDTA, heparin, and salt. This product is a new type of dual hot-start enzyme. Before high-temperature denaturation, the Taq enzyme antibody binds to the enzyme and inhibits its activity. At the same time, the primer is also blocked by the binding protein, thus inhibiting non-specific annealing of the primer or primer-dimer caused by primer dimer under low temperature conditions. non-specific amplification. This enzyme has 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but has no 3’-5’ exonuclease activity. The PCR product amplified using this product has an "A" base attached to the 3' end, so it can be cloned directly into the T vector. Activity definition: The amount of enzyme required to catalyze the incorporation reaction of 10 nmol dNTP into acid-insoluble substances within 30 minutes at 74°C is one unit.
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Intended Use Product features Basic parameters
Intended Use

This product is genetically modified hot-start enzyme based on common polymerase with added protective agent, 

which is highly tolerant to EDTA, heparin, salt and other PCR inhibitors.

Heparin, salt and other PCR inhibitors. This product is a new type of dual hot start enzyme, before denaturation at high temperature, 

Taq enzyme antibody binds to the enzyme and inhibits its activity.

Before denaturation at high temperature, Taq enzyme antibody binds to the enzyme and inhibits its activity, and at the same time, 

the primer is closed by the binding protein, thus inhibiting non-specific annealing or primer dimerisation of the primer under low temperature conditions.

This inhibits the non-specific annealing of primers or non-specific amplification caused by primer dimerisation at low temperature.


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Product features

Tolerance: High tolerance to EDTA and heparin.

Sensitivity: This product is a new dual hot-start TaqDNA polymerase with high sensitivity.

Specificity: Optimized PCR buffer components improve reaction specificity.


Basic parameters

Component

Specifications

Super HPTaq DNA polymerase

10 x PCR buffer Mg2+ plus

100μL/

1mL/

500μL/



5mL/

5mL/

10× 5mL/


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