Ready-to-Use PCR Master Mix (lyophilized) is a premixed lyophilized reagent for PCR efficient amplification,
which includes double-enclosed hot-start Super HTaq DNA polymerase, MgCl2 and dNTPs. When using,
only add DNA templates and primers (or probes) according to the required amount, and add PCR-grade
water until each reaction reaches 20µL, and then the PCR amplification reaction of the DNA sample can be started.
The 3' end of the PCR product amplified by this product has an "A" base attached, so it can be directly cloned into
T-Vector; the PCR product is also suitable for sequencing identification.
Conventional PCR or quantitative PCR
Such as Taqman fluorescence probe method, which can perfom rapid detection on DNA samples
Component | Specifications | Note |
One-step RT-qPCR Master Mix(with dNTPs,lyophilized) PCR-grade water | 6× 8T 1.5mL | 1T/tube |