Dieses Kit ist für den qualitativen In-vitro-Nachweis der Nukleinsäure des Parainfluenzavirus Typ 3 (PIV3) in menschlichen oropharyngealen Abstrichproben vorgesehen.
Dieses Kit ist für die Verwendung durch qualifiziertes und geschultes klinisches Laborpersonal vorgesehen, das speziell in den Techniken der Echtzeit-PCR und der molekularen In-vitro-Diagnoseverfahren geschult und geschult wurde.
Dieses Kit ist nur für die In-vitro-Diagnostik bestimmt.
This kit utilizes real-time fluorescent PCR detection technology.
This kit uses the highly conserved sequence HN gene in the PIV3 gene as the target region, and designs specific primers and TaqMan fluorescent probes. The fluorescein of PIV3 is FAM, the length of the PIV3 amplification fragment is 85bp, and the rapid detection of PIV3 in oropharyngeal swab samples is realized by performing PCR amplification in an amplification tube, and the quality control of the whole detection process is carried out by exogenous internal control and external quality controls (positive control and negative control). The exogenous internal control, a MS2 pseudoviral particle, is designed to monitor the whole process by designing specific primers and probes, with a fluorescein of ROX and an amplification fragment of 66bp for the internal control.
Items | Parameters |
Sample type | Oropharyngeal swab |
Specification | 48T/Kit |
PCR instruments | HC 1600; ABI 7500 |
LoD | 0.02TCID50/0.1mL |
Storage/Shelf life | -25℃to 8℃ away from light; 18 months |
Obtained certificates | CE; NMPA |